[Development of technology for the substance of poly[3-(3,4-dihydroxyphenyl) glyceric acid] from Symphytum asperum].

Comfrey (Symphytum L.) is used to treat bone fractures, tendon injuries, ulcer lesions of gastrointestinal tract. It promotes wound healing, accelerates exudates resorption in lungs and reduces joints' inflammation. In Georgian folk medicine, herbal remedies from comfrey are used to accelerate regeneration processes. Comfrey contains hepatotoxic and carcinogenic pyrrolizidine alkaloids, besides the main active ingredient is poly [3 - (3,4-dihydroxyphenyl) glyceric acid] (PDPGA). The aim of present work was to develop a technology for the substance - poly [3-(3,4dihydroxyphenyl) glyceric acid] (PDPGA) from comfrey stems, free of toxic pyrrolizidine alkaloids. During the investigation the optimal conditions for extraction and purification have been established: on the first stage pyrrolizidine alkaloids were removed from plant material by supercritical extraction; then the crude polysaccharides' fraction was obtained by water extraction (raw materials/extragent ratio was 1:15 at 90oC, the procedure was carried twice for 60 and 90 minutes). The isolation of the final product - PDPGA from crude polysaccharides' fraction was carried out by ultrafiltration on membrane filters. Based on the results of the investigation the technological scheme for the substance has been developed.

[Study on the separation process of pharmacological active total alkaloids from Chelidonium majus L. growing in Georgia].

In present article was studied the separation process of pharmacological active total alkaloids from Chelidonium majus L. growing in Georgia. Alkaloids were extracted from medicinal herbal material and separated by liquid extraction, diluents gas and a microfiltration through membrane equipment. The obtained A1, A2, A3 fractions were analyzed by GC/MS method; in all cases separation proceeds by the principle of extraction of the target alkaloids. It was concluded that the A1 is enriched with α and ß cryptopins, and protopin, but homochelidonine and chelidonine are in low contents. As accompanying alkaloid is identified dihydrosanguinarine as an artifact; the A2 is enriched with the maximum contents of stylopine and protopin, but the poor contents of chelidonine and homochelidonine; the A3 is enriched with α and ß cryptopins and maximum content of chelidonine. Extraction of alkaloids from Chelidonium majus L. proceeds selectively, but depending on a way of separation of the total alkaloids allows varying qualitative and quantitative consistence of the final product.

[Creation of phytovaleologic drugs].

This is a new field of knowledge in medical sciences that means "Healthy Way of Life". Based on the data of medical plants and on the results of Phytochemical and Pharmacological studies, carried out in preliminary experiments, there are cultivated compositions of Valeological substances, potentially having radio protective activity. As the basis for the selection of raw materials and their correlation in mixture, there were taken the data of spontaneous analysis of chemical composition having pharmacological (radio protective) activity of various compositions. As the result, the types of studied raw materials and their parties by weight in total mixture have been defined: Valeological substances 1: 1. Tea (leaves); 2. Common plantain (leaves); 3. Horse gowan (flowers); 4. Blackberry (leaves) 5. Hips; 6. Peppermint (leaves). Valeological substances 2: 1. Tea (leaves); 2. Dandelion (leaves); 3. Horse gowan (flowers); 4. Knotgrass (herbage); 5. Hips; 6. Peppermint (leaves) Experimental studies revealed that the most optimal way of brewing tea is rational method. Valeological substances 1 and 2 (tea with radio protective activity) "Protector" and "Adjara" - correspondingly. Technical conditions and technological instructions for radio protective tea "Protector" and "Adjara" are created. Pharmacological studies of tea "Protector" and "Adjara" have taken place in the Branch of Biological University of Buryatia (Academy of Science of Russian Federation) under the supervision of Prof. S. M. Nikolaev. Radio protective teas "Protector" and "Adjara" is approved by the Ministry of Health Sciences.

[Radio protective drug production from fresh leaves of Aloe arborescens Mill].

Nowadays, phytogenous drugs are wildly used as radio protective substances. The aim of the research was to study radio protective characteristics of aloe juice fraction and to develop new technology for radio protective drug production. Technological scheme for getting the drug in two stages. The first stage - extraction of juice from fresh leaves; the second stage - extracting bagasse have been developed and optimal environment for bagasse extraction are defined: Infusion of bagasse with 96 % ethyl spirit (1:1) during 30 minutes, continuation of extracting with water on correlation to raw materials 10:1 at temperature of 70 degrees C during 30 minutes. For the basis of the first series of balanced loading there are taken the optimal parameters of extracting process, on the basis of which in its turn was developed technological scheme of getting dry extract of aloe. Dry extract is a fine-dispersed reddish-yellow (brownish-yellow) powder, which can be easily dissolved in warm (40-60 degrees C) water. Pharmacological researches were conducted in the Institute of General and Experimental Biology, Siberian Branch, Russian. Academy of Sciences. The remarkable radio protective effect of the drug was revealed.

[Technology of vitamins containing double layer suppositories].

At present ascorbic acid and cyanocobalamin are widely used in medical practice. Often they are administered simultaneously; chemically they are incompatible, as ascorbic acid (redox compound) inactivates cyanocobalamin. The combination of ascorbic acid and cyanocobalamin is the problem of pharmacy. To solve the problem double-layer suppositories containing two incompatible vitamins: ascorbic acid and cyanocobalamin were developed. Pharmaceutical bioavailability of ascorbic acid and cyanocobalamin has been studied using in-vitro methods to develop optimal base for suppositories. As a result of investigations optimal composition and technology of the double-layer suppositories containing ascorbic acid and cyanocobalamin which met requirements has been developed. Sequential release of active compounds and as a result - sequential pharmacologic action is achieved.

[Development of inhalation powder formulation of gentamicin sulphate].

Aminoglycosides, including gentamicin, are considered among the most useful classes of antibiotics for treating Pseudomonas aeruginosa infections. The major drawback of aminoglycosides is the need for their relatively high-dose intravenous administration, which carries the potential for systemic toxicity. Granulometry analysis of gentamicin sulfate powder (substance) has shown, that generally it consists of large particles, and thus is not suitable for inhalation. Six different inhalation composition containing gentamicin sulfate has been developed, one of which showed the best inhalation properties. Technological chart and pilot Master formula for inhalation gentamicin sulfate composition delivered via Handi Haler have been developed.

[Perolen cream for therapy of rosacea].

Acne rosacea belongs to the most common inflammatory dermatoses of the face. Current treatments for this disease remain unsatisfactory, that's why searching for new methods and remedy are very important. For the topical therapy we have used a new cream perolen ("New Pharma", Georgia). The main component of the creme is the water extract of gastric mucous membrane of cow, which is the source of proteolytic ferments. Another main component is a Perilla oil which contains unsaturated fatty acids--linolenic and linol, presented in the highest amounts. The cream is characterized by bactericidal, bacteriostatical, anti-inflammatory and anti-oxidant effects, it also assists to tissue regeneration, make blood vessels more elastic and decreases penetration. We have observed 40 patients with papulo-pustular subtype of rosacea (25 females and 15 males). Patients were divided into two groups. For oral therapy in both groups we used Tetracycline and Trichopol. For topical treatment in the first group of patients was used rosamet cream and in the second group--perolen cream. Both of these creams were applied once daily during 2 months. Reduction of comedones, inflammations, papules, pustules and small nodules occurred after one month. Erythema, oedema, desquamation improved significantly during the treatment, but the positive effects were more prominent in the second group of patients. In conclusion, the study shows that topically used cream perelon represents a new approach in the management of acne rosacea.